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Nitrate reductase enzyme activity and free proline contents in sugarcane roots under water and acid stress in soil

机译：土壤水分和酸胁迫下甘蔗根系硝酸盐还原酶活性和脯氨酸含量。

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摘要

The aim of this work was evaluate the physiological indicators of association between water deficiency and soil acidity, by determining the nitrate reductase activity, the levels of free proline and initial growth of the root system of seedlings of sugarcane cv. IAC91-5155. After 30 days, the seedlings were transferred to plastic pots with 12 dm3 of dystrophic alic Red Latosol (Oxisol) and submitted to association of three treatments of water availability: no stress (70%), moderate stress (55%) and severe stress (40%), in according with field capacity and three acidity treatments: no stress (55%), moderate stress (33%) and severe stress (23%), considering the base saturation. The experimental design was that of random blocks under factorial scheme of 3x3, with four replicates. After 60 days under the stress association, the levels of free proline, the nitrate reductase activity and the growth of the sugarcane roots system were evaluated in seedlings of sugarcane. The nitrate reductase enzyme activity can be considered a physiological indicator of the effect of the association of acid and water stress in moderate conditions in soil, while the free proline can be considered physiological indicator to both stress in severe conditions. Water deficiency increasing reduced growth of sugarcane roots.
机译：这项工作的目的是通过确定硝酸盐还原酶活性，游离脯氨酸的水平和甘蔗树苗根系的初始生长来评估缺水与土壤酸度之间的生理指标。 IAC91-5155。 30天后，将幼苗转移至装有12 dm3营养不良的酸红色Latosol（Oxisol）的塑料盆中，并接受三种水处理方式的组合：无胁迫（70％），中度胁迫（55％）和严重胁迫（ 40％），并根据田间持水量和三种酸度处理：考虑到饱和度，无应力（55％），中度应力（33％）和重度应力（23％）。实验设计是3x3阶乘方案下的随机块的设计，具有四个重复。在逆境胁迫下60天后，评估了甘蔗幼苗中游离脯氨酸的水平，硝酸还原酶活性和甘蔗根系的生长。硝酸还原酶活性可以认为是中等条件下土壤中酸和水分胁迫相关影响的生理指标，而游离脯氨酸可以认为是严重条件下两种胁迫的生理指标。缺水增加了甘蔗根的生长。

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